Simona Ciriello

IGSDHD class 2010 associated student

AG Gehring

Photo of Simona  Ciriello

Simona graduated in 2010 in Naples University, Italy with the M.Sc. in Biotechnology. Since October 2011, she's been a PhD student at the Niels Gehring's lab associated to the IGSDHD program.

PhD title: Deciphering the functions of BTZ during mammalian nonsense-mediated mRNA decay

Nonsense mediated mRNA decay (NMD) is a gene expression surveillance mechanism that degrades transcripts with premature termination codons (PTCs). In mammals, the presence of an exon junction complex (EJC) downstream of a termination codon leads to the activation of NMD and degradation of the mRNA. The EJC is deposited on mRNAs during splicing and consists of four proteins: MAGOH, Y14, BTZ and eIF4A3. While MAGOH, Y14 and eIF4A3 are primarily localized to the nucleus, Barentsz (BTZ) is the only cytoplasmic core component.

Using different BTZ mutant I aim to identify the function of each domain, the specific interaction partners and the change in RNA-interactome.